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Title   HL-60 세포에서 CD18 과 Myeloperoxidase 유전자 발현에 대한 12-O-Tetradecanoylphorbol-13-Acetate 영향 ( Effects of 12-O-Tetradecanoylphorbol-13-Acetate on CD18 and Myeloperoxidase Genes Expression in HL-60 cells )
Publicationinfo   1995 Jan; 027(06): 1030-1040.
Key_word   CD18, Myeloperoxidase, HL-60, TPA
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Abstract   The CD18(￥a-subunit of integrin) is essential for expression of structural and functional CD11/CD18(￥a/￥a-subunit) complexes on the cell surface, and myeloperoxidase plays a role in the bacteriocidal action of granulocytes. Effects on CD18 and myeloperoxidase genes expression have been investigated during TPA-induced differentiation of HL-60 cells. CD18 mRNA was induced at 24 hr and myeloperoxidase mRNA was rapidly reduced at 12 hr. The level of CD18 mRNA was induced and myeloperoxidase was reduced in proportion to concentration of TPA. TPA-induced CD18 mRNA was slightly decreased and myeloperoxidase mRNA was no effect in cells treated with cycloheximide, respectively. On the other hand CD18 mRNA was completely reduced by actinomycin D pretreatment. TPA-dependent stimulation of CD18 gene and repression of myeloperoxidase gene were completely restored by staurosporin pretreatment. These results suggest that phosphorylation of a protein is a crucial step for the induction of CD18 mRNA and the repression of myeloperoxidase during TPA-induced differentiatian of HL-60.
저 자   임규(Kyu Lim),이종남(Jong Nam Lee),권광인(Kwang In Kweon),이명선(Myung Sun Lee),권기량(Gi Ryang Kweon),윤완희(Wan Hee Yoon),곽상태(Sang Tae Kwak),황병두(Byung Doo Hwang)